Journal: Cell Death & Disease

Article Title: Eradication of specific donor-dependent variations of mesenchymal stem cells in immunomodulation to enhance therapeutic values

doi: 10.1038/s41419-021-03644-5

Figure Lengend Snippet: A Three lineage differentiation of huMSCs and expression of cell surface markers. Upper Panel from left to right: MSCs differentiated to adipocytes, chondrocytes, and osteocytes. B BV2-suppression capacity and proliferation capacity of 32 lines of huMSCs. The black bars represent the ratio of BV2 cell mass after 48 h cultured in MSC-CM compared to RPMI-1640 (control medium). The red dots represent the ratio of total huMSC mass at 72 h after inoculation compared to 1 h after inoculation. C correlation between BV2-suppression capacity and huMSC proliferation capacity of 32 huMSC lines. D Boxplot shows BV2-suppression capacity between male ( n = 20) and female ( n = 20) huMSC lines, eight extra huMSC lines were added to increase the statistical power.

Article Snippet: The mouse microglia cell line BV2 was purchased from OBiO Technology (Shanghai) Corp. BV2 cells were cultured in RPMI-1640 supplemented with 20% FBS (Gibco) at 37 °C with saturated humidity and 5% CO 2 .

Techniques: Expressing, Cell Culture, Control

Journal: Cell Death & Disease

Article Title: Eradication of specific donor-dependent variations of mesenchymal stem cells in immunomodulation to enhance therapeutic values

doi: 10.1038/s41419-021-03644-5

Figure Lengend Snippet: A PCA of 32 huMSC lines. B Heatmap shows expression and clustering of genes, expression of which significantly correlated to BV2 inhibition capacity of 32 lines of huMSCs. C GO enrichment analysis of BV2 inhibition correlated genes. IFN-γ, autophagy, and related terms are highlighted in red, which are positively correlated with BV2 inhibition. Candidate genes belonging to each GO term are shown in blue. D GSEA analysis of enriched TFBS targeting gene sets, most of which, except for one, were positively correlated with BV2 inhibition (normalized enrichment score (NES) > 0). E Correlation between gene expression levels of two subunits of NF-κB complex and BV2 inhibition capacity. F Network shows connection between enriched GO terms, TFBS targeting gene sets, and positively correlated genes. Red squares represent GO terms and blue triangles represent TFBS targeting gene sets. The nodes of green balls are genes, which belonging to both corresponding GO terms and TFBS targeting gene sets.

Article Snippet: The mouse microglia cell line BV2 was purchased from OBiO Technology (Shanghai) Corp. BV2 cells were cultured in RPMI-1640 supplemented with 20% FBS (Gibco) at 37 °C with saturated humidity and 5% CO 2 .

Techniques: Expressing, Inhibition, Gene Expression

Journal: Cell Death & Disease

Article Title: Eradication of specific donor-dependent variations of mesenchymal stem cells in immunomodulation to enhance therapeutic values

doi: 10.1038/s41419-021-03644-5

Figure Lengend Snippet: A Anti-inflammation genes IDO1 , CXCL9 and IL6 were extensively up-regulated in huMSCs by stimulation of TNF-α and IFN-γ (2-factors). B In vitro analysis on BV2 inhibition showed elimination of donor-dependent variations in immune-modulations by two factors. C – E eradication of specific donor-dependent variations of huMSCs regarding treatment efficacies in LPS-induced neuroinflammation model, by open-field-motor scoring and hippocampal TNF-α expression. All data were normalized to LPS group, and were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The mouse microglia cell line BV2 was purchased from OBiO Technology (Shanghai) Corp. BV2 cells were cultured in RPMI-1640 supplemented with 20% FBS (Gibco) at 37 °C with saturated humidity and 5% CO 2 .

Techniques: In Vitro, Inhibition, Expressing

Journal: Cell Death & Disease

Article Title: Eradication of specific donor-dependent variations of mesenchymal stem cells in immunomodulation to enhance therapeutic values

doi: 10.1038/s41419-021-03644-5

Figure Lengend Snippet: A Venn diagram shows the overlap between genes up-regulated by 2-factor stimulation and genes positively correlated to BV2 inhibition capacity. B GO enrichment analysis of overlapped 227 genes. C GSVA heatmap shows specific GO-term associated gene sets in 32 huMSCs that positively or negatively correlated with BV2-cell immune suppressive capacity, and their expression levels before and after 2-factor stimulation. Results clearly indicated that interferon and NF-κB pathway associated genes were upregulated upon 2-factor stimulation, whereas genes related to autophagy and protein deacetylation did not correlate well with 2-factor stimulation.

Article Snippet: The mouse microglia cell line BV2 was purchased from OBiO Technology (Shanghai) Corp. BV2 cells were cultured in RPMI-1640 supplemented with 20% FBS (Gibco) at 37 °C with saturated humidity and 5% CO 2 .

Techniques: Inhibition, Expressing